A CRISPR system permits genetic engineering of essential genes and gene families.

Vyas VK, Barrasa MI, Fink GR
Sci Adv. 2015;1(3):e1500248. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
111429	pV1200	CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)
111428	pV1093	CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1
111427	pV1090	gRNA entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at RPS1 (Part of Duet system)
111425	pV1081	Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1
111424	pV1025	Expression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)
111423	pV1010	gRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)