N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli.
Sekar K, Gentile AM, Bostick JW, Tyo KE
PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.
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PubMed
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
65464 | pKS011 | pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry |
65465 | pKS012 | pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherry |
65466 | pKS013 | pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherry |
65467 | pKS014 | pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidase |
65468 | pJB005 | pTrc plasmid expressing GFP |
65469 | pJB028 | pTrc plasmid expressing p14*-TEV |
65470 | pKS042 | pTrc plasmid expressing clpA |
65471 | pKS043 | pTrc plasmid expressing clpS |
65472 | pKS044 | pTrc plasmid expressing clpP |
65915 | MG1655 Z1 malE | E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO |
65917 | MG1655 Z1 malE aat | E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, aat KO |
65918 | MG1655 Z1 malE clpA | E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, clpA KO |