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N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli.

Sekar K, Gentile AM, Bostick JW, Tyo KE
PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
65464pKS011pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry
65465pKS012pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and mCherry
65466pKS013pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing mCherry
65467pKS014pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidase
65468pJB005pTrc plasmid expressing GFP
65469pJB028pTrc plasmid expressing p14*-TEV
65470pKS042pTrc plasmid expressing clpA
65471pKS043pTrc plasmid expressing clpS
65472pKS044pTrc plasmid expressing clpP
65915MG1655 Z1 malEE. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO
65917MG1655 Z1 malE aatE. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, aat KO
65918MG1655 Z1 malE clpAE. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE, clpA KO

Antibodies from Article