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Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG.

Xu S, Chisholm AD
Sci Rep. 2016 Feb 10;6:21271. doi: 10.1038/srep21271. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
74111pCZGY2139Membrane targeted PH-miniSOG entry clone for efficient ablation of cells when expressed under desired promoter.
74112pCZGY2849Enhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.
87757pCZGY2141Pcol-19-PH::miniSOG, expression of membrane targeted PH-miniSOG in epidermis.
87758pCZGY2850Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.
87759pCZGY2838Pmyo-3-PH::miniSOG, expression of membrane targeted PH-miniSOG in body wall muscles.
87760pCZGY2844Punc-17b-PH::miniSOG, expression of membrane targeted PH-miniSOG in cholinergic motor neurons.
87761pCZGY2851Punc-17b-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in cholinergic motor neurons.
87762pCZGY2842Punc-25-PH::miniSOG, expression of membrane targeted PH-miniSOG in GABAergic motor neurons.
87763pCZGY2845Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.
87764pCZGY2840Pmec-4-PH::miniSOG, expression of membrane targeted PH-miniSOG in mechanosensory neurons
87765pCZGY2839Prgef-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in neurons.
90466pCZGY546Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

Antibodies from Article