A method for using direct injection of plasmid DNA to study cis-regulatory element activity in F0 Xenopus embryos and tadpoles.
Wang C, Szaro BG
Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010. Epub 2014 Nov 20.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 74100 | pBluescriptII[attB/Ins1] | "intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015) |
| 74101 | pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2] | vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015 |
| 74102 | pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2] | plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene) |