A Modular Assembly Platform for Rapid Generation of DNA Constructs.
Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68408 | TOPO 2-5 | TOPO backbone for GMAP genes with sites #2 and #5 |
| 68409 | TOPO 1-4 | TOPO backbone for GMAP promoters with sites #1 and #4 |
| 68410 | RV 2-5 | Retroviral backbone for use with GMAP |
| 68411 | LV 1-5 | Lentiviral backbone for use with GMAP |
| 68412 | R26TV CAG LSL 2-5 | Rosa26 targeting vector backbone for use with GMAP |
| 68413 | TOPO mTagBFP2 | TOPO construct expressing mTagBFP2 for generation of GMAP-compatible gene |
| 68441 | TOPO mKate2 | TOPO construct expressing mKate2 for generation of GMAP-compatible gene |
| 68442 | TOPO mKO2 | TOPO construct expressing mKusabira-Orange2 for generation of GMAP-compatible gene |
| 68443 | TOPO iRFP670 | TOPO construct expressing iRFP670 for generation of GMAP-compatible gene |
| 68444 | TOPO CloverCP | TOPO construct expressing CloverCP for generation of GMAP-compatible gene |
| 68445 | TOPO HygroR | TOPO construct expressing HygroR for generation of GMAP-compatible gene |
| 68446 | TOPO ZeoR | TOPO construct expressing ZeoR for generation of GMAP-compatible gene |
| 68447 | TOPO PuroR | TOPO construct expressing PuroR for generation of GMAP-compatible gene |
| 68448 | TOPO rtTA3-2A-Cre | TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene |
| 68449 | TOPO tTR-KRAB-rtTA3-Luc | TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene |
| 68450 | TOPO Cre-2A-GFP | TOPO construct expressing Cre-2A-GFP for generation of GMAP-compatible gene |
| 68451 | TOPO EFS | TOPO construct expressing EFS for generation of GMAP-compatible promoter |
| 68452 | TOPO pGK | TOPO construct expressing pGK for generation of GMAP-compatible promoter |
| 68453 | TOPO CCSP | TOPO construct expressing CCSP for generation of GMAP-compatible promoter |
| 68454 | TOPO CMV | TOPO construct expressing CMV for generation of GMAP-compatible promoter |
| 68455 | TOPO SV40 | TOPO construct expressing SV40 for generation of GMAP-compatible promoter |
| 68456 | TOPO UBC | TOPO construct expressing UBC for generation of GMAP-compatible promoter |
| 68457 | TOPO pTRE | TOPO construct expressing pTRE for generation of GMAP-compatible promoter |
| 68458 | TOPO HRE | TOPO construct expressing HRE for generation of GMAP-compatible promoter |
| 68464 | FFiBlast MSCV Puro | Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line |
| 68469 | MSCV Puro | Retroviral plasmid with multiple cloning site and puromycin resistance |
| 68470 | FFiBlast p5 | Inverted and floxed blasticidin resistance |
| 68471 | pTRE:shRNA A-pGK:rtTA-Cre | Inducible lentivirus expressing an arbitrary shRNA and Cre |
| 68475 | pGK:TRL-EFS:iRFP670 | Lentiviral plasmid expressing tTR-KRAB-rtTA3-Luc cassette and iRFP670 |
| 68476 | R26TV LSL-TRL | Rosa26 targeting vector expressing Cre-dependent tTR-KRAB-rtTA3-Luc cassette |
| 68477 | pTRE:iRFP670-EFS:Cre-2A-GFP | Inducible lentivirus expressing iRFP670, Cre, and GFP |
| 68478 | pTRE:CloverCP-EFS:ZeoR | Lentivirus for establishment of doxycycline-inducible cell line expression CloverCP |
| 68479 | pGK:HygroR-CMV:mTagBFP2-A_UTR | Lentivirus expressing mTagBFP2 with UTR sensor cassette and hygromycin resistance |
| 68480 | pGK:HygroR-CMV:mKate2-B_UTR | Lentivirus expressing mKate2 with UTR sensor cassette and hygromycin resistance |
| 68481 | pGK:HygroR-CMV:mKO2-C_UTR | Lentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance |
| 68482 | MSCV Puro-UBC:GFP | Retrovirus expressing GFP driven by UBC promoter |
| 68483 | MSCV Puro-SV40:GFP | Retrovirus expressing GFP driven by SV40 promoter |
| 68484 | MSCV Puro-EFS:GFP | Retrovirus expressing GFP driven by EFS promoter |
| 68485 | MSCV Puro-CMV:GFP | Retrovirus expressing GFP driven by CMV promoter |
| 68486 | MSCV Puro-pGK:GFP | Retrovirus expressing GFP driven by pGK promoter |
| 68487 | MSCV Puro-CCSP:GFP | Retrovirus expressing GFP driven by CCSP promoter |
| 68488 | TOPO GFP | TOPO construct expressing GFP for generation of GMAP-compatible gene |
| 68489 | TOPO CFP | TOPO construct expressing CFP for generation of GMAP-compatible gene |
| 68490 | TOPO mCherry | TOPO construct expressing mCherry for generation of GMAP-compatible gene |
| 68491 | TOPO mT2 | TOPO construct expressing mT2 for generation of GMAP-compatible gene |
| 68493 | TOPO Venus | TOPO construct expressing Venus for generation of GMAP-compatible gene |