A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species.
Nyerges A, Csorgo B, Nagy I, Balint B, Bihari P, Lazar V, Apjok G, Umenhoffer K, Bogos B, Posfai G, Pal C
Proc Natl Acad Sci U S A. 2016 Feb 16. pii: 201520040.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 72677 | pORTMAGE-2 | Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker. |
| 72678 | pORTMAGE-3 | Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker. |
| 72679 | pORTMAGE-4 | Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker. |
| 72680 | pORTMAGE-1 | Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme. |