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A Tunable Protein Piston That Breaks Membranes to Release Encapsulated Cargo.

Polka JK, Silver PA
ACS Synth Biol. 2016 Feb 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
71224Reb_1 aka PAS619Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone
71225Reb_79 aka PAS620Reb_1 with RebA(Q91R)
71226Reb_80 aka PAS621Reb_1 with RebB(I67T, N84I)
71227Reb_81 aka PAS622Reb_1 with RebB(T28A, D72G)
71228Reb_82 aka PAS623Reb_1 with RebB(S82P, D88G)
71229Reb_83 aka PAS624Reb_1 with RebA(G53A, D89G)
71230Reb_140 aka PAS625Reb_1 with RebA(S88P)
71231Reb_65 aka PAS626Reb_1 with RebA(Y82A)
71232Reb_67 aka PAS627Reb_1 with RebA(S88A)
71233Reb_221 aka PAS628Reb_1 with RebA(G90A)
71234Reb_206 aka PAS629Expresses wild type R bodies with mNeonGreen-tagged second copy of RebB under a weak RBS downstream as well as soluble mCherry
71235Reb_207 aka PAS630Reb_206 with RebA(S88P)

Antibodies from Article