Optogenetic control of nuclear protein export.
Niopek D, Wehler P, Roensch J, Eils R, Di Ventura B
Nat Commun. 2016 Feb 8;7:10624. doi: 10.1038/ncomms10624.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 72655 | NLS-mCherry-LEXY (pDN122) | Blue light-inducible nuclear export of mCherry |
| 72656 | pLEXY (pDN137) | golden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS) |
| 72657 | pNLS-LEXY (pDN138) | golden gate entry vector for mCherry-LEXY tagging of proteins (N-terminal cMyc(P1A) NLS) |
| 72658 | H2B-GFP-LEXY (pDN157) | Constitutively nuclear LEXY variant for blocking endogenous, CRM-1-dependent nuclear export with blue light |
| 72659 | H2B-GFP-AsLOV2-NES27 (pDN158) | Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export |
| 72660 | NLS-mCherry-NES reporter (pDN160) | NLS-mCherry-NES 17 reporter for light-inducible nuclear export block with pDN157 and pDN158 |
| 72661 | LEXY TR (without KRAB domain) (pDN162) | LexATF-T2A-NLS-LexA-mCherry-LEXY construct for light control of transcriptional repression |
| 72662 | LEXY TR (with KRAB domain) (pDN163) | LexATF-T2A-NLS-LexA-KRAB-mCherry-LEXY construct for light control of transcriptional repression |