ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes.
Yoshimi K, Kunihiro Y, Kaneko T, Nagahora H, Voigt B, Mashimo T
Nat Commun. 2016 Jan 20;7:10431. doi: 10.1038/ncomms10431.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 72602 | pCas9-polyA | express hCas9 byT7 promoter with polyadenine tails |