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Assessment of costimulation and coinhibition in a triple parameter T cell reporter line: Simultaneous measurement of NF-kappaB, NFAT and AP-1.

Jutz S, Leitner J, Schmetterer K, Doel-Perez I, Majdic O, Grabmeier-Pfistershammer K, Paster W, Huppa JB, Steinberger P
J Immunol Methods. 2016 Jan 15. pii: S0022-1759(16)30007-2. doi: 10.1016/j.jim.2016.01.007. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
118031pSIRV-NFAT-eGFPFluorescent reporter for NFAT activation
118094pSIRV-NF-kB-eCFPFlourescent reporter for NF-kB activity
118095pSIRV-AP-1-mCherryFluorescent reporter for AP-1 activation

Antibodies from Article