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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

Ho SM, Hartley BJ, Tcw J, Beaumont M, Stafford K, Slesinger PA, Brennand KJ
Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. doi: 10.1016/j.ymeth.2015.11.019. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
79049pLV-TetO-hNGN2-PuroExpresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.
79823pLV-TetO-hNGN2-eGFP-PuroExpresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

Antibodies from Article