Insights into Ubiquitination from the Unique Clamp-like Binding of the RING E3 AO7 to the E2 UbcH5B.
Li S, Liang YH, Mariano J, Metzger MB, Stringer DK, Hristova VA, Li J, Randazzo PA, Tsai YC, Ji X, Weissman AM
J Biol Chem. 2015 Dec 18;290(51):30225-39. doi: 10.1074/jbc.M115.685867. Epub 2015 Oct 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 137840 | pCDNA3.1+ HA-hAO7 | N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells |
| 137842 | pGEX6p1 AO7R | GST tagged AO7 encoding aa 126-216 for expression in bacteria |
| 137843 | pGEX6p1 AO7RE | GST tagged AO7 encoding aa 126-258 for expression in bacteria |
| 139318 | pETDUET-GST AO7RE ∆238-245 | GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria |
| 139319 | pETDUET-GST AO7RE I137A | GST tagged AO7 encoding aa 126-258 with an I137A mutation for expression in bacteria |
| 139427 | pETDUET-GST AO7RE P199A | GST tagged AO7 encoding aa 126-258 with a P199A mutation for expression in bacteria |
| 139428 | pETDUET-GST AO7RE Y165A | GST tagged AO7 encoding aa 126-258 with a Y165A mutation for expression in bacteria |
| 213935 | pETDUET-GST AO7REwt | GST tagged AO7 encoding aa 126-258 for expression in bacteria |