Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA.
Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE
Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71820 | pCR1002 | Express Streptococcus pyogenes Cas9 |
| 71821 | pCR1003 | Express Streptococcus pyogenes dCas9 (D10A, H840A) |
| 71822 | pCR1054 | Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS |
| 71823 | pCR1055 | Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS |
| 71824 | pCR1056 | Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two C-terminal SV40 NLS |
| 71825 | BFP dest clone | Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression) |