Pathway engineered enzymatic de novo purine nucleotide synthesis.
Schultheisz HL, Szymczyna BR, Scott LG, Williamson JR
ACS Chem Biol. 2008 Aug 15;3(8):499-511. doi: 10.1021/cb800066p.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
73623 | pET22-HT/purC | Produces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tag |
73624 | pET22-HT/prsA | Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag |
73625 | pET22-HT/plsA | Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag |
73631 | pET22-HT/spoR | Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag |