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Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx.

Dynes JL, Amcheslavsky A, Cahalan MD
Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 10.1073/pnas.1523410113. Epub 2015 Dec 28. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
73561G-GECO1-Orai1Green fluorescent reporter of Orai1-associated calcium influx
73562G-GECO1.2-Orai1Green fluorescent reporter of Orai1-associated calcium influx
73563G-GECO1-Orai3Green fluorescent reporter of Orai3-associated calcium influx
73564Orai1-GCaMP6fGreen fluorescent reporter of Orai1-associated calcium influx
73565G-GECO1.2-Orai1Y80EGreen fluorescent reporter of mutant Orai1Y80E-associated calcium influx
73566mCherry-CADSoluble red fluorescent activator of Orai1 channels
73567CMV-CADSoluble activator of Orai1 channels

Antibodies from Article