Programmable polyproteams built using twin peptide superglues.

Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, Howarth M
Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
72327	pET21 MBPx-SpyCatcher	Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.
72326	AviTag-SpyCatcher	Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.
72325	pET28a SnoopTag-mEGFP-SpyTag	Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.
72324	pET28a SpyCatcher-SnoopCatcher	Bacterial expression of SpyCatcher linked to SnoopCatcher.
72323	pET28a SnoopTag-MBP	Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.
72322	pET28a-SnoopCatcher	Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.