Evolution of translation machinery in recoded bacteria enables multi-site incorporation of nonstandard amino acids.
Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW, Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Soll D, Rinehart J, Isaacs FJ
Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. Epub 2015 Nov 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73544 | pEvol-pAcFRS.2.t1 | tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG |
| 73545 | pEvol-pAcFRS.1.t1 | tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG |
| 73546 | pEvol-pAzFRS.2.t1 | tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG |
| 73547 | pEvol-pAzFRS.1.t1 | tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG |
| 73581 | C321.deltaA (Isaacs lab) | Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene |