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Fluorogenic Green-Inside Red-Outside (GIRO) Labeling Approach Reveals Adenylyl Cyclase-Dependent Control of BKalpha Surface Expression.

Pratt CP, He J, Wang Y, Barth AL, Bruchez MP
Bioconjug Chem. 2015 Sep 16;26(9):1963-71. doi: 10.1021/acs.bioconjchem.5b00409. Epub 2015 Sep 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
73212pcDNA3.1-kappa-HA-dL5-BKaExpresses HA-dL5(E52D)-BKa (KCNMA1, mSlo alpha subunit) in mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP)
73213pcDNA3.1-BKaExpresses BKa (KCNMA1, mSlo alpha subunit) in mammalian cells. (tagless, leaderless)

Antibodies from Article