High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.

Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J
Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
74190	pLCKO_Luciferase_sgRNA	lentiviral vector expressing sgRNA targeting Luciferase
74188	pLCKO_EIF3D_sgRNA_4	lentiviral vector expressing sgRNA targeting EIF3D
74187	pLCKO_EIF3D_sgRNA_1	lentiviral vector expressing sgRNA targeting EIF3D
74185	pLCKO_PSMB2_sgRNA_5	lentiviral vector expressing sgRNA targeting PSMB2
74184	pLCKO_PSMB2_sgRNA_1	lentiviral vector expressing sgRNA targeting PSMB2
74181	pLCKO_PSMD1_sgRNA_5	lentiviral vector expressing sgRNA targeting PSMD1
74180	pLCKO_PSMD1_sgRNA_1	lentiviral vector expressing sgRNA targeting PSMD1
74179	pLCKO_LacZ_sgRNA	lentiviral vector expressing sgRNA targeting LacZ
73311	pLCKO	Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.
73310	Lenti‐Cas9‐2A‐Blast	Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter