Mammalian polymerase theta promotes alternative NHEJ and suppresses recombination.

Mateos-Gomez PA, Gong F, Nair N, Miller KM, Lazzerini-Denchi E, Sfeir A
Nature. 2015 Feb 12;518(7538):254-7. doi: 10.1038/nature14157. Epub 2015 Feb 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
73131	pX330(puro) Rosa26-H3F3B	Expression in Mammalian Cells, Cas9 Nuclease to analize translocations
73132	pcDNA 3.1(Hygro) myc-hPolQ-Flag	To express in mammalian cells human PolQ