A Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells.
Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 72851 | pSK23 | template for C-terminal eGFP tagging, Puromycin-resistance |
| 72852 | pSK24 | template for C-terminal Twin-Strep tag, Puromycin-resistance |
| 72853 | pSK25 | template for C-terminal double-FLAG tag, Puromycin-resistance |
| 72854 | pSK32 | template for C-terminal TEV-site + V5-tag, Puromycin-Resistance |
| 72855 | pSK15 | template for insertion of attP site (C-terminal tag plasmid design), Blasticidin-Resistance |
| 72856 | pKF292 | template base vector for N-terminal tagging, inducible Blasticidin resistance |
| 72857 | pKF293 | template for N-terminal 3x FLAG tag, inducible Blasticidin resistance |
| 72858 | pKF294 | template for N-terminal 1x Strep tag, inducible Blasticidin resistance |
| 72859 | pKF290 | template for N-terminal Strep-8xHis-Strep-TEV site tag, inducible Blasticidin resistance |
| 72860 | pRB30 | template for N-terminal eGFP tag, inducible Blasticidin resistance |
| 72861 | pRB36 | template base vector for N-terminal tagging, constitutive Blasticidin resistance |
| 72862 | pRB34 | template for N-terminal 3x FLAG tag, constitutive Blasticidin resistance |
| 72863 | pRB32 | template for N-terminal 1x Strep tag, constitutive Blasticidin resistance |
| 72864 | pRB38 | template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Blasticidin resistance |
| 72865 | pRB40 | template for N-terminal eGFP tag, constitutive Blasticidin resistance |
| 72866 | pRB35 | template base vector for N-terminal tagging, constitutive Puromycin resistance |
| 72867 | pRB33 | template for N-terminal 3x FLAG tag, constitutive Puromycin resistance |
| 72868 | pRB31 | template for N-terminal 1x Strep tag, constitutive Puromycin resistance |
| 72869 | pRB37 | template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance |
| 72870 | pRB39 | template for N-terminal eGFP tag, constitutive Puromycin resistance |
| 72871 | pKF295 | expression of improved FLP recombinase |
| 74773 | pKF296 | template for C-terminal TEV-site + 2x V5-tag (short version), Blasticidin-Resistance |
| 74774 | pKF297 | template for C-terminal TEV-site + 2x V5-tag (short version), Puromycin-Resistance |
| 74886 | pSK41 | template for insertion of attP site (C-terminal tag plasmid design), Puromycin-Resistance |
| 74887 | pIS1 | targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C |