Engineered acetoacetate-inducible whole-cell biosensors based on the AtoSC two-component system.
Rutter JW, Dekker L, Fedorec AJH, Gonzales DT, Wen KY, Tanner LES, Donovan E, Ozdemir T, Thomas GM, Barnes CP
Biotechnol Bioeng. 2021 Nov;118(11):4278-4289. doi: 10.1002/bit.27897. Epub 2021 Aug 9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 72839 | PatoG-OG241 | Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone. |
| 72840 | PatoK-OG241 | NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone. |
| 72841 | PatoL-OG241 | Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone. |
| 72842 | PatoM-OG241 | Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone. |
| 72846 | PatoL-LOG241 | Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone. |
| 72847 | PatoM-LOG241 | Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. LOG241 backbone. |
| 72848 | PatoK-LOG241 | NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone. |