MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.

Sakuma T, Nakade S, Sakane Y, Suzuki KT, Yamamoto T
Nat Protoc. 2016 Jan;11(1):118-33. doi: 10.1038/nprot.2015.140. Epub 2015 Dec 17. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
63670	pX330S-2-PITCh	Expresses Cas9 nuclease and the PITCh-gRNA
63671	pX330A-FBL/PITCh	Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA
63672	pCRIS-PITChv2-FBL	PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus