SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly.

Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, Dushek O, van der Merwe PA, Howarth M
J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. Epub 2014 Aug 21. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
59549	Traptavidin-E6	pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography
59548	Dead Streptavidin-SpyTag	pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers
59547	Dead Streptavidin-SpyCatcher	pET21-DCatch encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyCatcher, for generation of chimeric SpyAvidin tetramers