Automated physics-based design of synthetic riboswitches from diverse RNA aptamers.
Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63848 | AEB-No-aptamer-controlmRFP1 | This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression. |
| 63849 | AEB-F12 | This pFTV1 vector contains a fluoride binding riboswitch, controlling the translation initaition rate of mRFP1 gene. |
| 63850 | AEB-Theo45 | This pFTV1 vector contains a theophylline binding riboswitch, controlling the translation initaition rate of mRFP1 gene. |
| 63851 | AEB-No-aptamer-control -- AEB3mRFP1 | This pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene, using a weak AEB3 promoter. It should be used when testing AEB-DNT3 riboswitch to measure the non-specific DNT effect on mRFP1 expression. |
| 63852 | AEB-DNT3 | This pFTV1 vector containsa 2,4,dinitrotoluene (DNT) binding riboswitch, controlling the translation initaition rate of mRFP1 gene. |
| 63853 | AEB-No-aptamer-controlluciferase | This pFTV1 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-Dopa5, AEB-T4-2 riboswitches to measure their non-specific ligand effect on luciferase expression. |
| 63854 | AEB-Dopa5 | This pFTV1 vector contains a dopamine binding riboswitch, controlling the translation initaition rate of luciferase gene. |
| 63855 | AEB-T4-2 | This pFTV1 vector contains a thyroxine binding riboswitch, controlling the translation initaition rate of luciferase gene. |
| 63856 | Mishler2014-No-aptamer-controlluciferase-pUC19 | This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression. |
| 63857 | AEB-TMR7pUC19 | This pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene. |
| 63858 | AEB-TMR10pUC19 | This pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene. |
| 71419 | AEB-Theo27 pUC19 | This pUC19 vector contains a theophylline binding riboswitch, controlling the translation initiation rate of luciferase gene. |