Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress.
Jensen SI, Lennen RM, Herrgard MJ, Nielsen AT
Sci Rep. 2015 Dec 8;5:17874. doi: 10.1038/srep17874.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
68122 | pSIJ8 | Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression |
68246 | Strain SIJ_488 | E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI. |