T. J. Kappock Lab: Additional purEK associated constructs
Kappock
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 72440 | pJK521 | Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX) |
| 72442 | pJK523 | Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX) |
| 72444 | pJK536 | Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX) |
| 72445 | pJK538 | Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1) |
| 72446 | pJK540 | Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK) |
| 72447 | pJK541 | Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK) |
| 72449 | pJK547 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F) |
| 72450 | pJK550 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins) |
| 72451 | pJK552 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F) |
| 72452 | pJK553 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1) |
| 72453 | pJK554 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1) |
| 72454 | pJK565 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Ala/Trp165>Phe mutant (AaPurE1fwf-H59A) |
| 72455 | pJK569 | Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene |
| 72456 | pJK573 | Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene |
| 72457 | pJK578 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Ala/Trp165>Phe mutant (AaPurE1fwf-S57A) |
| 72458 | pJK585 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Cys/Trp165>Phe mutant (AaPurE1fwf-S57C) |
| 72459 | pJK586 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Thr/Trp165>Phe mutant (AaPurE1fwf-S57T) |
| 72460 | pJK616 | Produces Treponema denticola ATCC 35405 PurE with an N-terminal His6 tag (H6TdPurE2) |
| 72461 | pJK626 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F) |
| 72462 | pJK627 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F) |
| 72463 | pJK628 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala/Tyr154>Phe mutant (AaPurE1-S57A,Y154F) |
| 72464 | pJK629 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F) |
| 72465 | pJK632 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val/Tyr154>Phe mutant (AaPurE1-S57V,Y154F) |
| 72466 | pJK636 | Produces Treponema denticola ATCC 35405 PurE with a TEV protease-cleavable N-terminal His6 tag (H6tTdPurE2) |
| 73371 | pJK292 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N) |
| 73372 | pJK293 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F) |
| 73373 | pJK294 | Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D) |
| 73494 | pJK322 | Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutase |
| 73495 | pJK323 | Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide synthetase |