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In vitro studies of DNA mismatch repair proteins.

Geng H, Du C, Chen S, Salerno V, Manfredi C, Hsieh P
Anal Biochem. 2011 Jun 15;413(2):179-84. doi: 10.1016/j.ab.2011.02.017. Epub 2011 Feb 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
72300pSCW01Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
72301pSCW02Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
129423pFB1_hMSH2Expression human MSH2 protein using baculovirus
129424pFB1_hMSH6Expression human MSH6 protein using baculovirus
129425pFB1_hPMS2Expression human PMS2 protein using baculovirus
129426pFB1_hMLH1Expression human MLH1 protein using baculovirus
134874pFB1_hMSH2_G674AExpression human MSH2 G674 mutant protein using baculovirus
135466pFB HTB_hMSH6_T1219DExpression human MSH6_T1219D mutant protein using baculovirus

Antibodies from Article