Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease.
Lawrenson T, Shorinola O, Stacey N, Li C, Ostergaard L, Patron N, Uauy C, Harwood W
Genome Biol. 2015 Nov 30;16(1):258. doi: 10.1186/s13059-015-0826-7.
(Link opens in a new window)
PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68252 | pICSL11055 | Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette |
| 68253 | pICSL11057 | Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley |
| 68254 | pICSL11058 | Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley |
| 68255 | pICSL11061 | Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea |
| 68256 | pICSL11062 | Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleracea |
| 68257 | pICSL12009 | Level 0 Golden Gate Part: Promoter and 5UTR Ubiquitin (Zea mays) |
| 68258 | pICSL11056 | Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants |
| 68259 | pICSL80036 | Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli) |
| 68260 | pICSL80037 | Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli) |
| 68261 | pICSL90002 | Level 0 Golden Gate Part: Promoter U6-26 (Arabidopsis thaliana) |
| 68262 | pICSL90003 | Level 0 Golden Gate Part: Promoter U6 (Triticum aestivum) |
| 68263 | pICSL11059 | Level 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassette |
| 68264 | pICSL11060 | Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea) |