A set of powerful negative selection systems for unmodified Enterobacteriaceae.

Khetrapal V, Mehershahi K, Rafee S, Chen S, Lim CL, Chen SL
Nucleic Acids Res. 2015 Jul 27;43(13):e83. doi: 10.1093/nar/gkv248. Epub 2015 Mar 23. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
73163	pSLC-217	Template plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.
73189	pSLC-242	Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
73190	pSLC-246	Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
73191	pSLC-237	Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.
73192	pSLC-238	Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
73193	pSLC-239	Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
73194	pSLC-241	Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection.