A versatile method for cell-specific profiling of translated mRNAs in Drosophila.
Thomas A, Lee PJ, Dalton JE, Nomie KJ, Stoica L, Costa-Mattioli M, Chang P, Nuzhdin S, Arbeitman MN, Dierick HA
PLoS One. 2012;7(7):e40276. doi: 10.1371/journal.pone.0040276. Epub 2012 Jul 6.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
71729 | pUAST-GFP-RpL10Ab | Expression of Drosophila melanogaster Rpl10Ab-GFP fusion |
125223 | pUAST-attB-3xHA-V5-RpL10Ab | 3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression |