The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans.
Zhang L, Ward JD, Cheng Z, Dernburg AF
Development. 2015 Nov 9. pii: dev.129635.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 69490 | pJW1354 | Gly-Ser-4xGly linker-degron-TEV-3xFLAG with 40 bp nhr-25 homology arms |
| 71719 | pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR) | Expressing a GFP-tagged degron in the soma of C. elegans |
| 71720 | pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR) | Expressing an mRuby-tagged TIR1 in the soma of C. elegans |