A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression.
Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, Cech TR
Genome Biol. 2015 Nov 10;16(1):231. doi: 10.1186/s13059-015-0791-1.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71390 | FLAG-LoxP-PURO-LoxP-SNAP-TERT | Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection. |
| 71391 | FLAG-LoxP-GFP-LoxP-SNAP-TERT | Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection. |
| 71397 | HR-TERT-SV40-GFP | Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm. |
| 71398 | HR-TERT-promotor | Homologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm. |