Optogenetic acidification of synaptic vesicles and lysosomes.
Rost BR, Schneider F, Grauel MK, Wozny C, G Bentz C, Blessing A, Rosenmund T, Jentsch TJ, Schmitz D, Hegemann P, Rosenmund C
Nat Neurosci. 2015 Nov 9. doi: 10.1038/nn.4161.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 70111 | pLenti-Synapsin-pHoenix-WPRE | lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles |
| 70112 | pCMV-lyso-pHoenix | Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes |
| 70113 | pCMV-lyso-pHluorin | Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification |