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Addgene

A general system for generating unlabelled gene replacements in bacterial chromosomes.

Leenhouts K, Buist G, Bolhuis A, ten Berge A, Kiel J, Mierau I, Dabrowska M, Venema G, Kok J
Mol Gen Genet. 1996 Nov 27;253(1-2):217-24. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
71595pORI28EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.
71852EC1000RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

Antibodies from Article