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An active site-tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase.

Murphy JR, Donini S, Kappock TJ
Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1292-9. doi: 10.1107/S2053230X15015939. Epub 2015 Sep 23. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
71590pJK438Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag (TpCSH6)
71591pJK511Produces Thermoplasma acidophilum citrate synthase with C-terminal His6 tag, His222>Gln mutant (TpCSH6-H222Q)

Antibodies from Article