Crystal structures of Acetobacter aceti succinyl-coenzyme A (CoA):acetate CoA-transferase reveal specificity determinants and illustrate the mechanism used by class I CoA-transferases.
Mullins EA, Kappock TJ
Biochemistry. 2012 Oct 23;51(42):8422-34. doi: 10.1021/bi300957f. Epub 2012 Oct 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71575 | pJK512 | Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E) |
| 71576 | pJK513 | Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A) |
| 71577 | pJK514 | Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A) |
| 71578 | pJK516 | Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q) |
| 71579 | pJK517 | Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D) |
| 71580 | pJK524 | Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A) |
| 71581 | pJK564 | Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A) |