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The partial substrate dethiaacetyl-coenzyme A mimics all critical carbon acid reactions in the condensation half-reaction catalyzed by Thermoplasma acidophilum citrate synthase.

Kurz LC, Constantine CZ, Jiang H, Kappock TJ
Biochemistry. 2009 Aug 25;48(33):7878-91. doi: 10.1021/bi9006447. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
71573pJK381Produces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)
71574pJK382Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)

Antibodies from Article