Metabolic engineering of Escherichia coli using CRISPR-Cas9 meditated genome editing.
Li Y, Lin Z, Huang C, Zhang Y, Wang Z, Tang YJ, Chen T, Zhao X
Metab Eng. 2015 Sep;31:13-21. doi: 10.1016/j.ymben.2015.06.006. Epub 2015 Jun 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71538 | pCas9cur | Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli. |
| 71539 | pGRB | for expression of gRNA in E. coli |
| 71541 | pREDCas9 | Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli. |