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Mycobacterial recombineering.

Murphy KC, Papavinasasundaram K, Sassetti CM
Methods Mol Biol. 2015;1285:177-99. doi: 10.1007/978-1-4939-2450-9_10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
71486pKM342Contains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.
71487pKM343Contains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.
107692pNitET-SacB-kanExpression of the phage Che9c RecET system for Recombineering in mycobacteria
107706pCre-SacB-zeoExpresses Cre recombinase for excising chromosomal loxP-Hyg-loxP cassette inserts.
107770pKM402Expression of phage Che9c RecT for oligo-mediated recombineering in mycobacteria

Antibodies from Article