Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration.
Ng CY, Farasat I, Maranas CD, Salis HM
Metab Eng. 2015 May;29:86-96. doi: 10.1016/j.ymben.2015.03.001. Epub 2015 Mar 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 63847 | pCN-mBFP | Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged. |
| 71420 | CYN2/150 (E. coli Entner-Doudoroff v11) | This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway with an improved NADPH regeneration rate. Module is Cm resistant |
| 71421 | CYN1 (E. coli Entner-Doudoroff v1) | This E. coli MG1655 strain has a genome-integrated genetic module containing a rationally engineered 5-enzyme Entner-Doudoroff pathway (first version). The module also contains a Cm resistance marker. |