ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo.
Zentner GE, Kasinathan S, Xin B, Rohs R, Henikoff S
Nat Commun. 2015 Oct 22;6:8733. doi: 10.1038/ncomms9733.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 70231 | pGZ108 (pFA6a-3FLAG-MNase-kanMX6) | ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope |
| 70232 | pGZ109 (pFA6a-3FLAG-MNase-HIS3MX6) | ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA- HIS3MX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope |
| 70233 | pGZ110 (pFA6a-3FLAG-MNase-TRP1) | ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tag |
| 70234 | pGZ173 (pFA6a-SL-MNase-kanMX6) | ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope |
| 72273 | pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS) | FLAG-Mnase expressed under the REB1 promoter |