Targeted methylation and gene silencing of VEGF-A in human cells by using a designed Dnmt3a-Dnmt3L single-chain fusion protein with increased DNA methylation activity.
Siddique AN, Nunna S, Rajavelu A, Zhang Y, Jurkowska RZ, Reinhardt R, Rots MG, Ragozin S, Jurkowski TP, Jeltsch A
J Mol Biol. 2013 Feb 8;425(3):479-91. doi: 10.1016/j.jmb.2012.11.038. Epub 2012 Dec 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71827 | pET28-Dnmt3a3L-sc27 | For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker. |