Stabilization of exosome-targeting peptides via engineered glycosylation.
Hung ME, Leonard JN
J Biol Chem. 2015 Mar 27;290(13):8166-72. doi: 10.1074/jbc.M114.621383. Epub 2015 Feb 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 71292 | pcDNA Flag Lamp2b-HA | Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b. |
| 71293 | pcDNA GNSTM-3-Flag-10-Lamp2b-HA | Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag. |
| 71294 | pcDNA GNSTM-3-RVG-10-Lamp2b-HA | Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag. |
| 71295 | pcDNA GASTM-3-RVG-10-Lamp2b-HA | Encodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag. |