The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing in Escherichia coli.
Reisch CR, Prather KL
Sci Rep. 2015 Oct 14;5:15096. doi: 10.1038/srep15096.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62654 | pKDsgRNA-ack | Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression |
| 62655 | pCas9-CR4 | Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR |
| 62656 | pKDsgRNA-p15 | Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid |