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Biochemical and structural studies of N5-carboxyaminoimidazole ribonucleotide mutase from the acidophilic bacterium Acetobacter aceti.

Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, Francois JA, Goodman RA, Kappock TJ
Biochemistry. 2006 Jul 11;45(27):8193-208. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
71129pJK130Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli
71132pJK175Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli
71134pJK278Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli
71135pJK279Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli
71136pJK280Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli
71137pJK281Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli
71138pJK282Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli
71139pJK283Expression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coli
71140pJK314Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli
71141pJK315Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli
71142pJK318Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli
71143pJK319Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli
71144pJK320Expression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coli
71831pJK173Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE, and purK genes
71832pJK348Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genes
71833pJK347Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genes
71834pJK193Cloned region contains Acetobacter aceti 1023 rpmE-radC(and beyond) region
71835pJK174Expression of Acetobacter aceti 1023 PurE and PurK in E. coli
71836pJK346Cloned region contains Acetobacter aceti 1023 purE(H59D) gene
71837pJK340Cloned region contains Acetobacter aceti 1023 purE(H59N) gene
71838pJK324Cloned region contains Acetobacter aceti 1023 purE gene

Antibodies from Article