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Rapid cell-free forward engineering of novel genetic ring oscillators.

Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, Maerkl SJ
Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
70716pET21a(+)-Histag-Cerulean (526) Expression vector for Cerulean purification
70718pET21a(+)-Histag-Citrine (525)Expression vector for Citrine purification
70719pET21a(+)-Histag-mCherry (527)Expression vector for mCherry purification
70723pBetI-BCD7-Citrine P69U18C68T11 v40-1 (532)Citrine reporter for transfer fxns
70725pLacI-BCD7-Citrine P42U18C68T11 v40-1 (536)Citrine reporter for transfer fxns
70726pCI-BCD7-Citrine P62U18C68T11 v40-1 (523)Citrine reporter for transfer fxns
707273n1 (572)BetI/PhlF/SrpR BCD7 3-node v41-1
707305n1 (560)TetR/PhlF/SrpR/LambdaCI/LacI BCD7 5-node v41-2
70731pBetI-BCD7-phlF-ssrA P69U18C51T15 v40-3 (570)plasmid for building 3n1
70732pBetI-BCD7-qacR-ssrA P69U18C63T16 v50-2 (568)plasmid for building 5n2
70733pLacO1-BCD7-tetR-ssrA P42U18C45T13 v40-5a (497)plasmid for building 5n1
70734pSrpR-BCD7-betI-ssrA P65U18C64T14 v50-2 (567)plasmid for building 3n1, 5n2
70735pSrpR-BCD7-lambdaCI-ssrA P65U18C47T14 v40-2 (495)plasmid for building 5n1
70736pSrpR-BCD7-tetR-ssrA P65U18C45T14 v40-2 (491)plasmid for building 3n2
70737pCI-BCD7-lacI-ssrA P62U18C50T16 v40-4a (496)plasmid for building 5n1
70739pQacR-BCD7-tetR-ssrA P68U18C45T13 v40-5 (569)plasmid for building 5n2
70740pTetR-BCD7-phlF-ssrA P66U18C51T15 v40-3a (492)plasmid for building 3n2, 5n1, 5n2
70741pPhlF-BCD20-sfGFP-ssrA P64U23C39T13 v1-1 (462)1 color strong reporter used in study
70742pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)1 color weak reporter used in study
70743pPhlF-BCD20-Citrine-ssrA P64U23C88T18 v1-1 (586)for building 3-color reporter plasmid
70744pSrpR-BCD20-Cerulean-ssrA P65U23C89T19 v50-2a (587)for building 3-color reporter plasmid
70745pTetR-BCD20-mCherry-ssrA P66U23C90T20 v40-3a (588)for building 3-color reporter plasmid

Antibodies from Article