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The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish.

Walton EM, Cronan MR, Beerman RW, Tobin DM
PLoS One. 2015 Oct 7;10(10):e0138949. doi: 10.1371/journal.pone.0138949. eCollection 2015. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
70052p5E-mfap45' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.
135210p3E tdTomatoRed fluorescent tag
135211p3E p2a-tomatoRed fluorescent tag
135214pDEST mfap4:tdTomatoRed tagged macrophages
135217pDEST mfap4:icre-p2a-tomatoCre coexpressed with tdTomato in macrophages
135218pDEST mfap4:turquoise2Cyan tagged macrophages
188700p3e tdTomatoGateway 3' element encoding tdTomato
188701pDEST tol2 UbB polyAGateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence
188702p3e UbB polyAGateway 3' element encoding the ubiquitin B polyadenylation sequence

Antibodies from Article