The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish.
Walton EM, Cronan MR, Beerman RW, Tobin DM
PLoS One. 2015 Oct 7;10(10):e0138949. doi: 10.1371/journal.pone.0138949. eCollection 2015.
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PubMed
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
70052 | p5E-mfap4 | 5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes. |
135210 | p3E tdTomato | Red fluorescent tag |
135211 | p3E p2a-tomato | Red fluorescent tag |
135214 | pDEST mfap4:tdTomato | Red tagged macrophages |
135217 | pDEST mfap4:icre-p2a-tomato | Cre coexpressed with tdTomato in macrophages |
135218 | pDEST mfap4:turquoise2 | Cyan tagged macrophages |
188700 | p3e tdTomato | Gateway 3' element encoding tdTomato |
188701 | pDEST tol2 UbB polyA | Gateway destination vector containing the zebrafish ubiquitin B polyadenylation sequence |
188702 | p3e UbB polyA | Gateway 3' element encoding the ubiquitin B polyadenylation sequence |