Conditional protein splicing of alpha-sarcin in live cells.
Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 2014 Jan 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 70223 | pcDNA3-FRB-VMAc-CSarcin | Expresses VMA intein with C-terminal portion of alpha-sarcin |
| 70225 | pcDNA3-NGFP-VMAn-FKBP | Expresses N-terminal portion of GFP along with VMA intein for protein splicing |
| 70226 | pcDNA3-FRB-VMAc-CGFP | Expresses C-terminal portion of GFP along with VMA intein for protein splicing |
| 70229 | 3XFLAG-NSarQ27G-VMAn-FKBP | Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing |
| 70230 | NSarQ27G-VMAn-FKBP | Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing |