Linear DNA for rapid prototyping of synthetic biological circuits in an Escherichia coli based TX-TL cell-free system.
Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. Epub 2013 Dec 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 45784 | pBEST-p15a-PLtetO-1-UTR1-LacI-T500 | |
| 45833 | pBADmod1-linker2-gamS | |
| 70191 | pBEST_OR2-OR1-Pr-UTR1_ClpX-T500 (48p) | Derived from 21 with “ClpX” coding sequence from Genbank U00096.3 substituted for “deGFP” – used as T500 template for 134-137. |
| 70194 | pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p) | Derived from 21 with silent mutations to make BsaI, BbsI compatible |
| 70195 | pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p) | Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector |
| 70197 | pBEST-p15A-OR2-OR1-Pr-UTR1-deGFPT500 (121p) | Promoter panel |
| 70198 | pBEST-p15A-Pl-tetO1-deGFP-T500 (122p) | Promoter panel |
| 70199 | pBEST-p15A-Pl-lacO1-deGFP-T500 (123p) | Promoter panel |
| 70200 | pBEST-p15A-J23113-deGFP-T500 (124p) | Promoter panel |
| 70201 | pBEST-p15A-J23114-deGFP-T500 (125p) | Promoter panel |
| 70202 | pBEST-p15A-J23116-deGFP-T500 (126p) | Promoter panel |
| 70203 | pBEST-p15A-J23150-deGFP-T500 (127p) | Promoter panel |
| 70204 | pBEST-p15A-J23106-deGFP-T500 (128p) | Promoter panel |
| 70205 | pBEST-p15A-J23151-deGFP-T500 (129p) | Promoter panel |
| 70206 | pBEST-p15A-J23100-deGFP-T500 (130p) | Promoter panel |
| 70207 | pBEST-p15A-J23101-deGFP-T500 (131p) | Promoter panel |
| 70208 | pBEST-p15A-J23102-deGFP-T500 (132p) | Promoter panel |
| 70209 | pBEST-p15A-pNull-deGFP-T500 (133p) | Promoter panel |
| 70210 | P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p) | 1 Part of 4-part switch |
| 70211 | P4U2C8T2-v1-1 (Pl-tetO1-deCFP) (135p) | 1 Part of 4-part switch |
| 70212 | P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p) | 1 Part of 4-part switch |
| 70213 | P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p) | 1 Part of 4-part switch |